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Protocols

Protocols are step-by-step workflows for calibrations. Use the Protocols page in the UI to run a calibration protocol, enter the required metadata, and follow the guided prompts.

Protocols page in the Pioreactor UI

Run a calibration protocol​

  1. Open Protocols from the left navigation.
  2. Choose the protocol that matches your calibration goal.
  3. Enter a clear name and any metadata requested by the flow.
  4. Follow the on-screen steps to collect measurements.
  5. At the review step, check the recorded points and fitted curve. Correct entered values or uncheck points to exclude them, then select Update chart to refit before saving.
  6. Save the calibration and set it active if needed.

Review is available for OD600 standards, duration-based pump, DC-based stirring, and fusion OD protocols. Stirring calibrations run on all Pioreactors at once keep every reading; CLI calibrations save as before.

The available protocols depend on connected hardware and the calibration type you select. For optical density calibrations, see Standard curves for OD600 readings.