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Activities

Below is a list of built-in activities that come with the Pioreactor. Additionally, you can add activities using plugins. Read more about using plugins.

  • Stirring: starts the stirring in the Pioreactor. After starting, you can change the stirring speed in the Settings tab. You can modify the default stirring speed in the configuration settings. We recommend keeping the stirring range between 150 RPM and 600 RPM.

  • Optical density: turns on the system that measures culture density. Readings will start to populate the Experiment Overview page. It's useful to inspect the first few minutes to ensure the readings look reasonable (for example, they aren't erratic and the stirring speed is appropriate). Read more about optical density and normalization.

  • Growth Rate: this activity requires that stirring and optical density be running as well. It starts the calculation of a normalized optical density and the implied growth rate. Both calculations will populate the Experiment Overview page. Read more about growth-rate modelling.

  • Temperature automation: turning on a temperature automation will start collecting temperature readings. For example, the Thermostat automation will use a feedback loop to stabilize the Pioreactor to a set temperature. Pausing an automation will cease both any active heating and temperature data collection. Stopping an automation will stop the heating and temperature collection. To change a running temperature automation, use the "Change Temperature Automation" button. Read more about specific temperature automations.

  • Dosing automation: turning on a dosing automation starts listening periodically for dosing triggers. A dosing automation requires at least 1 pump to be available and calibrated. More about using pumps. Pausing a dosing automation will stop any current and future dosing until the automation is unpaused. Read more about the different types of dosing automations.

  • LED automation: turning on an LED automation starts listening periodically for LED change triggers. Read more about LED automations.