# Using a peristaltic pump with your Pioreactor

You can attach up to three peristaltic pumps to your Pioreactor, typically used for: adding fresh media to the vial, removing effluent from the vial, and adding an alternative media to the vial.

First decided what you want this pump to do: add media, remove waste, or add alternative media. Pumps that are supplied by us will have a polarized connector that connects to the PWM outputs on the Pioreactor hat. Which PWM you use is determined by your PWM settings in config.ini.

In our case, if we were to use the pump as a media pump, we would connect the pump to PWM channel 2.

## Attaching the pumps

1. Insert the cable of one peristaltic pump into PWM channel 2. This is your media pump.
2. Insert the cable of the other pump into PWM channel 4. This is your waste pump.

More details on attaching pumps can be found here.

note

The peristaltic pump has two tubes: a source and a sink. Source tubes take up liquid, and sink tubes expel liquid. You can label your tubes with coloured tape as we have in the images.

tip

Supplying external power? Make sure to plug in your external power before pump calibration!

## Calibrating the pumps

2. Type ssh pioreactor@<insert unit name>.local.
• For example, to calibrate on our Pioreactor named worker3, we typed ssh pioreactor@worker3.local.
• The default password is raspberry.
3. Type pio run pump_calibration.
4. Follow the prompts to calibrate your media, waste, and/or alternate media pumps.
5. Calibrations can be performed depending on the frequency of your Pioreactor use.

## Sterilizing the pumps​

To avoid cross contamination, pumps should be sterilized before and after use.

1. Create a 10% bleach dilution in a beaker. Place the sink and source tubes of each pump you're using in the beaker.
2. On the UI, in the Pioreactors tab, click Manage and go to the Dosing tab.
3. Run continuously to cycle the disinfectant solution through each pump that you're using.
4. Interrupt after sufficient cycling. In another beaker with deionized water, place the tubes in and repeat the cycling to rinse out the pumps.
5. Your pumps are now ready to be attached to media, waste, or vials for your experiments.

Once finished calibrating, you can run your pump manually and programmatically.

### Manually from the command line​

pio run add_media --ml 3

or, if you wish to run continuously until interrupted.

pio run add_media --continuously

## Programmatically run pumps using automations​

Dosing automations, like turbidostats, chemostats, fed-batch and more, are available to run once your pumps are attached and calibrated. Read more about automations.