Gather the 20ml vial, stirbar, and stainless steel ports.
Remove the cap from the vial.
Gently but firmly push the stainless steel ports into the four holes. The two straight ports are placed in the outer holes, and the two bent ports are placed in the inner holes.
The O-rings are pre-lubricated with a silicone-based lubricant. If needed, remove any excess lubricant from the O-rings.
The top vial holder has a groove within the body where the thinner #024 O-ring sits. Carefully insert the O-ring using your fingers. Make sure to not twist the O-ring.
Run your finger inside the vial holder, pushing the O-ring into place to ensure a snug fit.
The bottom vial holder has a groove for the thicker #121 O-ring. The O-ring will sit between the base of the holder and the heater PCB. Carefully insert the O-ring using your fingers. Make sure to not twist the O-ring.
Insert the other end of the flex cable below the O-ring and into the groove. Pull it through.
Apply pressure on the back of the heater PCB so that it lies flat on the O-ring. Maintain this pressure for the next steps.
While maintaining pressure, flip the holder and insert the two M2.5-CS 5mm screws into the holes - don't tighten fully yet.
While maintaining pressure, take turns screwing each in until both are reasonably tight, and the heater PCB is securely attached to the bottom vial holder.