More on peristaltic pumps
The PWM output default to 5V (specifically, it's tied to the power supply, so it may be 5.1V, 5.2V etc. See your power supply). You are able to use 12V pumps successfully even at 5V output. However, you can also use the AUX power connection to change the PWM output.
Note that the inner tubing on pumps is often different from the outer tubing. For example, for the peristaltic pumps that we supply:
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Interior tubing dimensions: 3.3mm OD, 1mm ID
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Exterior tubing dimensions: 1/8" OD, 1/16" ID
Pumps that we have used and we like:
note
These pumps require some assembly to work with the Pioreactor